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FV peptide induces apoptosis in HEp 2 and HeLa cells: an insight into the mechanism of induction

Balasubashini, Sri M and Karthigayan, S and Somasundaram, ST and Balasubramanian, T and Rukkumani, R and Menon, Venugopal P (2006) FV peptide induces apoptosis in HEp 2 and HeLa cells: an insight into the mechanism of induction. Journal of Carcinogenesis, 5 (27).

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Abstract

The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa) from lionfish (Pterios volitans) venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml) at different time points (12, 24 and 36 hrs) were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.

Item Type: Article
Subjects: Oceanography > marine biology
Divisions: Annamalai Univ - Marine Biology
Depositing User: CASMB
Date Deposited: 03 Mar 2010 04:00
Last Modified: 03 Mar 2010 04:00
URI: http://moeseprints.incois.gov.in/id/eprint/39

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