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Biochemistry of TBT-degrading marine pseudomonads isolated from Indian coastal waters

Sampath, R and Venkatakrishnan, H and Ravichandran, V and Chaudhury, RR (2012) Biochemistry of TBT-degrading marine pseudomonads isolated from Indian coastal waters. Water, Air, and Soil Pollution, 223 (1). pp. 99-106.

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Abstract

Tributyltin (TBT) is a very effective biocide and an active ingredient in antifouling paints. Screening along the Indian coast yielded 49 bacterial isolates capable of TBT assimilation. The screening was done based on the ability of bacteria to grow in mineral salt medium (MSM) containing TBT as the sole source of carbon. All the isolates produced exopolysaccharides (biosurfactants) in the medium which aid in emulsification and thus ease bioavailability of TBT. Five isolates were identified as potent TBT degraders (namely, Pseudomonas pseudoalcaligenes, Pseudomonas stutzeri, Pseudomonas mendocina, Pseudomonas putida, and Pseudomonas balearica) based on their biomass production in MSM containing TBT as the sole source of carbon. In addition to evaluating the potential of individual bacterial strains, the study also focused on using a consortium of bacteria to explore their synergistic effect when grown on TBT. Further tests like growth profile, rhamnolipid secretion profile, extracellular protein secretion profile, and detection of siderophores were performed on these isolates when grown in MSM supplemented with 2 mM TBT concentration. Emulsification activity of the crude extracellular polysaccharides against kerosene was evaluated. It can be therefore inferred that TBT degradation by these marine pseudomonads is a twostep process: (a) dispersion of TBT in the aqueous phase and (b) tin-carbon bond cleavage by siderophores affecting debutylation of TBT. The consortium of bacteria may be effective in the treatment of TBT-contaminated waste water in dry docks.

Item Type: Article
Additional Information: Copyright of this article belongs to Springer.
Uncontrolled Keywords: Debutylation; Pseudomonas putida; Rhamnolipids; Siderophores; Tributyltin chlorides, Antifouling paint; Biochemistry; Carbon; Chlorine compounds; Docks; Emulsification; Physiology; Polysaccharides; Sewage; Surface active agents; Tin; Wastewater; Wastewater treatment, Bacteria, carbon; rhamnolipid; siderophore; tin; tributyltin, bacterium; bioavailability; biochemistry; biodegradation; chemical bonding; coastal water; lipid; polysaccharide; tributyltin; waste treatment; wastewater, article; bacterial strain; bacterium identification; bacterium isolate; bacterium isolation; bioavailability; biochemistry; biomass production; carbon source; chemical analysis; coastal waters; controlled study; culture medium; degradation kinetics; emulsion; geographic distribution; growth rate; India; intermethod comparison; microbial consortium; microbial degradation; molecular dynamics; molecular interaction; nonhuman; physical chemistry; protein secretion; Pseudomonas; Pseudomonas balearica; Pseudomonas mendocina; Pseudomonas pseudoalcaligenes; Pseudomonas putida; Pseudomonas stutzeri, Bacteria (microorganisms); Pseudomonas balearica; Pseudomonas mendocina; Pseudomonas pseudoalcaligenes; Pseudomonas putida; Pseudomonas stutzeri
Subjects: Oceanography > oceanography
Depositing User: NIOT Library
Date Deposited: 19 Dec 2013 11:17
Last Modified: 19 Dec 2013 11:17
URI: http://moeseprints.incois.gov.in/id/eprint/251

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