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In vitro anticancer activity of natural β-carotene from Dunaliella salina EU5891199 in PC-3 cells

Jayappriyan, KR and Rajkumar, R and Venkatakrishnan, V and Nagaraj, S and Rengasamy, R (2013) In vitro anticancer activity of natural β-carotene from Dunaliella salina EU5891199 in PC-3 cells. Biomedicine and Preventive Nutrition, 3 (2). pp. 99-105.

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Abstract

Green microalga, Dunaliella, isolated from the saltpan of Tamil Nadu, India, was identified both morphologically and genetically via 18S rDNA specific primers. Amplified 18S rDNA of Dunaliella salina were sequenced and submitted in the GenBank, NCBI. The natural β-carotene from optimally grown cells was purified by column chromatography, which was confirmed by Thin Layer Chromatography (TLC) with the Retardation factor value of 0.92. It was also quantified by High Performance Liquid Chromatography (HPLC) with a quantity of 3. mg/mL of culture. The extracted β-carotene was assessed for its anticancer property in the human prostate cancer cell line (PC-3) which showed the effective apoptosis of 32 than the synthetic β-carotene which is 27 in flow cytometry analysis. Cytochrome c and caspase assay were also revealed that algal β-carotene mediated cell death was more compared to standard β-carotene. The Indian isolate, D. salina EU5891199 that has a potential effect on PC-3 cells has been documented in the present study.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier.
Uncontrolled Keywords: beta carotene; caspase 3; caspase 8; cytochrome c, antineoplastic activity; antiproliferative activity; apoptosis; article; cancer cell culture; column chromatography; concentration response; controlled study; DNA fragmentation; drug cytotoxicity; Dunaliella salina; enzyme activity; enzyme assay; flow cytometry; high performance liquid chromatography; human; human cell; male; priority journal; prostate cancer; thin layer chromatography, algae; Dunaliella; Dunaliella salina
Subjects: Oceanography > oceanography
Depositing User: NIOT Library
Date Deposited: 19 Dec 2013 12:12
Last Modified: 19 Dec 2013 12:12
URI: http://moeseprints.incois.gov.in/id/eprint/246

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